Plant Transcription Factor Database
Previous version: v3.0
Ziziphus jujuba
HSF Family
Species TF ID Description
XP_015868935.1HSF family protein
XP_015868967.1HSF family protein
XP_015870139.1HSF family protein
XP_015873555.1HSF family protein
XP_015875693.1HSF family protein
XP_015878058.1HSF family protein
XP_015881318.1HSF family protein
XP_015881319.1HSF family protein
XP_015881320.1HSF family protein
XP_015881322.1HSF family protein
XP_015884313.1HSF family protein
XP_015884394.1HSF family protein
XP_015888126.1HSF family protein
XP_015888349.1HSF family protein
XP_015888350.1HSF family protein
XP_015889252.1HSF family protein
XP_015889253.1HSF family protein
XP_015890148.1HSF family protein
XP_015891386.1HSF family protein
XP_015891434.1HSF family protein
XP_015891435.1HSF family protein
XP_015896232.1HSF family protein
XP_015897552.1HSF family protein
XP_015898083.1HSF family protein
XP_015898109.1HSF family protein
XP_015898214.1HSF family protein
XP_015898330.1HSF family protein
XP_015898712.1HSF family protein
XP_015901407.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645